5JCY
Spir2-GTBM bound to MyoVa-GTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979340 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.610, 41.220, 108.220 |
| Unit cell angles | 90.00, 115.89, 90.00 |
Refinement procedure
| Resolution | 44.806 - 1.800 |
| R-factor | 0.1533 |
| Rwork | 0.151 |
| R-free | 0.19130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lx1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.092 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.810 | 1.860 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 39553 | |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 50 mM Hepes, 50 mM MOPS, 10% (w/v) PEG 1000, 10% (w/v) PEG 3350, 10% (v/v) MPD |
