5JCV
Sortase B from Listeria monocytogenes.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 70.155, 70.155, 224.471 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.600 - 2.230 |
R-factor | 0.2087 |
Rwork | 0.207 |
R-free | 0.24870 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.560 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.600 | 2.270 |
High resolution limit [Å] | 2.230 | 2.230 |
Rmerge | 0.087 | 0.890 |
Number of reflections | 14055 | |
<I/σ(I)> | 36.4 | 2.88 |
Completeness [%] | 99.2 | 100 |
Redundancy | 10.9 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2 M lithium sulfate, 2 M ammonium sulfate, 0.1 M Tris-Cl |