5JCE
Crystal structure of OsCEBiP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-04 |
| Detector | AGILENT EOS CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.448, 99.771, 87.647 |
| Unit cell angles | 90.00, 103.73, 90.00 |
Refinement procedure
| Resolution | 39.160 - 2.510 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jcd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.278 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 2.600 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Number of reflections | 33694 | |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 97.6 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1 M Tris, 2.0 M (NH4)2SO4 |
