5JCD
Crystal structure of OsCEBiP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-06 |
Detector | AGILENT EOS CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.772, 77.274, 111.391 |
Unit cell angles | 90.00, 99.02, 90.00 |
Refinement procedure
Resolution | 29.600 - 2.400 |
R-factor | 0.223 |
Rwork | 0.220 |
R-free | 0.27600 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.403 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.600 | 2.480 |
High resolution limit [Å] | 2.390 | 2.400 |
Number of reflections | 31051 | |
<I/σ(I)> | 22 | |
Completeness [%] | 98.3 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | 0.2 M ammonium acetate, 0.1 M sodium acetate trihydrate, 30%(w/v) PEG4000 |