5JBY

Lys27-linked triubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-28
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	51.758, 51.749, 54.977
Unit cell angles	90.02, 118.15, 119.95

Refinement procedure

Resolution	22.571 - 1.987
R-factor	0.2414
Rwork	0.237
R-free	0.30760
RMSD bond length	0.018
RMSD bond angle	2.331
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	22.571
High resolution limit [Å]	1.987
Number of reflections	22678
<I/σ(I)>	6.84
Completeness [%]	79.6
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% (w/v) PEG 3350, 4mM CdCl2, pH 5.9, 200mM Mg(NO3)2