5JBS
Conformational changes during monomer-to-dimer transition of Brucella suis VirB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.503, 78.723, 70.840 |
Unit cell angles | 90.00, 111.56, 90.00 |
Refinement procedure
Resolution | 42.200 - 1.950 |
R-factor | 0.19347 |
Rwork | 0.191 |
R-free | 0.24097 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.900 |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.200 |
High resolution limit [Å] | 1.950 |
Number of reflections | 48835 |
<I/σ(I)> | 2.4 |
Completeness [%] | 97.0 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 0.1 M Tris/HCl pH 6.8, 18% PEG-MME 2000 |