5JBM

Crystal structgure of Cac1 C-terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-10-10
Detector	RDI CMOS_8M
Wavelength(s)	1.0
Spacegroup name	P 41 2 2
Unit cell lengths	58.850, 58.850, 97.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.425 - 3.000
R-factor	0.2374
Rwork	0.233
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ejo
RMSD bond length	0.007
RMSD bond angle	0.920
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.010
High resolution limit [Å]	2.900	2.900
Rmerge	0.099	0.557
Number of reflections	4117
<I/σ(I)>	12.9	1.7
Completeness [%]	99.3	99.7
Redundancy	6.42	6.63

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	291	0.2 M sodium formate, 14% PEG 3350, 0.1 M Tris, pH 8.3