5JBL
Structure of the bacteriophage T4 capsid assembly protease, gp21.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.745, 124.041, 88.959 |
| Unit cell angles | 90.00, 89.96, 90.00 |
Refinement procedure
| Resolution | 41.869 - 1.943 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.20470 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.852 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.980 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.097 | 0.540 |
| Number of reflections | 61875 | |
| <I/σ(I)> | 11.9 | 2 |
| Completeness [%] | 93.0 | 76 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.3 M Ammonium sulfate, 0.1 M Hepes pH 7.5, and 0.1 M NaCl |
