5JBJ
Crystal structure of chicken LGP2 with 5'p 12-mer dsRNA at 3.6 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 64 |
Unit cell lengths | 90.090, 90.090, 196.730 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 78.020 - 3.580 |
R-factor | 0.25647 |
Rwork | 0.253 |
R-free | 0.31881 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jb2 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.954 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.020 | 3.730 |
High resolution limit [Å] | 3.580 | 3.580 |
Rmerge | 0.067 | 1.180 |
Number of reflections | 10663 | |
<I/σ(I)> | 20.74 | |
Completeness [%] | 78.0 | 99 |
Redundancy | 11.51 | 10.32 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | chLGP2, directly after size exclusion chromatography, was mixed with dsRNA in a 1:1 ratio and incubated for 30 minutes on ice. The complex was concentrated with an Amicon Ultra concentrator to around 10 mg/ml and then 2 mM ADP:AlF4 and 2 mM MgCl2 were added. chLGP2:12bp dsRNA:ADP:AlF4 complex was mixed with reservoir buffer (0.1 M Bis-Tris propane pH 6.5, 0.05-0.1 M magnesium formate) in a 2:1 ratio |