5JAV
Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91D at cryogenic temperature
Replaces: 5EGZExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-03-17 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.178, 60.353, 38.425 |
| Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
| Resolution | 38.350 - 1.895 |
| R-factor | 0.1939 |
| Rwork | 0.191 |
| R-free | 0.24480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.938 |
| Data reduction software | CrysalisPro (1.171.36.32) |
| Data scaling software | CrysalisPro (1.171.36.32) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.895 | 1.895 |
| Rmerge | 0.020 | 0.128 |
| Number of reflections | 11143 | |
| <I/σ(I)> | 48.99 | 5.93 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.3 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 26% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
