5JAJ
Structure of chicken LGP2 witha 5'p 10-mer dsRNA and ADP-AlF4-Mg.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.590, 97.050, 122.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.080 - 1.500 |
| R-factor | 0.13483 |
| Rwork | 0.133 |
| R-free | 0.16847 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TMI |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.510 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.100 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.746 | |
| Number of reflections | 132907 | |
| <I/σ(I)> | 17.6 | 2.31 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.25 | 5.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 5prime monophosphate 10-mer dsRNA:ADP:AlF4:mg complex was mixed with reservoir buffer (0.1 M tri-sodium citrate dihydrate pH 6.1, 0.04-0.08 M ammonium dihydrogen phosphate) in a 2:1 ratio. |
