5J9V
Ten minutes iron loaded Rana Catesbeiana H' ferritin variant E57A/E136A/D140A
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-05 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000, 1.722, 1.759 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 183.744, 183.744, 183.744 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.150 - 1.160 |
R-factor | 0.12856 |
Rwork | 0.128 |
R-free | 0.13827 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j8s |
RMSD bond length | 0.008 |
RMSD bond angle | 1.250 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.950 | 1.220 |
High resolution limit [Å] | 1.160 | 1.160 |
Rmerge | 0.062 | 0.354 |
Number of reflections | 91331 | |
<I/σ(I)> | 15.4 | 4.5 |
Completeness [%] | 99.8 | 100 |
Redundancy | 8.5 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 281 | 1.6-2.0 M MgCl2 and 0.1 M bicine pH 9.0 |