5J9U
Crystal structure of the NuA4 core complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2014-04-20 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 217.909, 189.889, 173.558 |
| Unit cell angles | 90.00, 113.61, 90.00 |
Refinement procedure
| Resolution | 29.686 - 2.950 |
| R-factor | 0.2162 |
| Rwork | 0.216 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.694 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.950 |
| Number of reflections | 106917 |
| <I/σ(I)> | 10.3 |
| Completeness [%] | 78.4 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | 100mM NaCitrate (pH 6.5), 1.79M ammonium sulfate, 5%(w/v) glycerol |
