5J9P

KcsA in vitro

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	80
Detector technology	CMOS
Collection date	2014-02-09
Detector	RDI CMOS_8M
Wavelength(s)	1
Spacegroup name	I 4
Unit cell lengths	155.695, 155.695, 74.791
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.638 - 2.850
R-factor	0.2216
Rwork	0.217
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.962	50.962	3.000
High resolution limit [Å]	2.850	9.010	2.850
Rmerge		0.034	1.976
Rmeas	0.202	0.037	2.132
Rpim	0.073	0.014	0.793
Total number of observations	160009	5023	22269
Number of reflections	21104
<I/σ(I)>	11.7	47.3	1.1
Completeness [%]	100.0	99.6	100
Redundancy	7.6	7.1	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG400