5J9N

tRNA guanine Transglycosylase (TGT) in co-crystallized complex with 2-(methylamino)-1H-benzo[d]imidazole-5-carbohydrazide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	C 1 2 1
Unit cell lengths	90.359, 64.547, 71.273
Unit cell angles	90.00, 92.85, 90.00

Refinement procedure

Resolution	41.681 - 1.640
R-factor	0.2145
Rwork	0.213
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p0d
RMSD bond length	0.006
RMSD bond angle	0.830
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1-2155_1492))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.120	1.740
High resolution limit [Å]	1.640	1.640
Rmerge		0.342
Number of reflections	49307
<I/σ(I)>	9.28	2.01
Completeness [%]	97.5	95.2
Redundancy	3	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	288	13% PEG 8000,100mM MES, 1mM DTT, 10% DMSO,