5J9M
tRNA guanine Transglycosylase (TGT) in co-crystallized complex with 2-(methylamino)-1H-benzo[d]imidazole-5-carboxamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.895 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.971, 64.946, 70.662 |
| Unit cell angles | 90.00, 96.30, 90.00 |
Refinement procedure
| Resolution | 45.210 - 1.330 |
| R-factor | 0.1308 |
| Rwork | 0.129 |
| R-free | 0.15710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p0d |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.808 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1-2155_1492: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.210 | 1.410 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.045 | 0.489 |
| Number of reflections | 93288 | |
| <I/σ(I)> | 14.3 | 2.3 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | 13% PEG 8000,100mM MES, 1mM DTT, 10% DMSO |
