5J9K

Crystal structure of the rice Topless related protein 2 (TPR2) N-terminal topless domain (1-209) in complex with rice D53 repressor EAR peptide motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 21 21 2
Unit cell lengths	57.718, 59.638, 171.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.377 - 2.550
R-factor	0.2166
Rwork	0.215
R-free	0.25280
RMSD bond length	0.006
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.550	2.550
Rmerge	0.090	0.949
Number of reflections	19882
<I/σ(I)>	14.6	2
Completeness [%]	99.4	99.2
Redundancy	8	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	25% w/v Polyethylene glycol 3350, 0.2 M Ammonium sulfate, 0.1 M BIS-TRIS, pH 6.5