5J9H
Crystal structure of Glycoprotein C from Puumala virus in the post-fusion conformation (pH 8.0)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | I 21 3 |
Unit cell lengths | 138.521, 138.521, 138.521 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.000 - 2.500 |
R-factor | 0.211 |
Rwork | 0.208 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j81 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.271 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.000 | 2.530 | |
High resolution limit [Å] | 2.500 | 20.000 | 2.500 |
Rmerge | 0.072 | 0.042 | 0.783 |
Number of reflections | 15391 | ||
<I/σ(I)> | 14.43 | 31.82 | 2.15 |
Completeness [%] | 99.6 | 86.8 | 99.6 |
Redundancy | 5.5 | ||
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 40% PEG 400 0.2 M Lithium sulphate 0.1 M Tris buffer pH 8.0 |