5J9A
Ambient temperature transition state structure of arginine kinase - crystal 11/Form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 298 |
| Detector technology | CMOS |
| Collection date | 2015-05-06 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.038, 72.047, 80.237 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.023 - 1.997 |
| R-factor | 0.1383 |
| Rwork | 0.135 |
| R-free | 0.17940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m15 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.496 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.023 | 2.050 |
| High resolution limit [Å] | 1.997 | 1.997 |
| Rmerge | 0.104 | 0.594 |
| Number of reflections | 26097 | |
| <I/σ(I)> | 32.9 | 1.9 |
| Completeness [%] | 98.0 | 88 |
| Redundancy | 6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 25 mM HEPES, pH 7.5 18% PEG 6000 |
