5J98

Crystal structure of Slow Bee Paralysis Virus at 2.6A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.990
Spacegroup name	I 2 2 2
Unit cell lengths	340.042, 396.848, 431.697
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.500 - 2.600
R-factor	0.279
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j96
Data reduction software	XDS (ai)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.500	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.200	0.980
Number of reflections	780730
<I/σ(I)>	6	0.9
Completeness [%]	88.3	69.3
Redundancy	6.8	0.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	Sodium acetate pH 4.5, 5% (v/v) PEG-10,000