5J97
Dimerization domain of cytoplasmic activation/proliferation-associated protein-2 (caprin-2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 71.020, 71.020, 133.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.160 - 2.550 |
R-factor | 0.214 |
Rwork | 0.211 |
R-free | 0.24400 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.990 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX (1.8.2_1309) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.158 | |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 11231 | |
<I/σ(I)> | 19.5 | |
Completeness [%] | 96.6 | |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20% PEG8000, 0.1 M Tris (pH8.0), 10% glycerol |