5J96

Crystal structure of Slow Bee Paralysis Virus at 3.4A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-01
Detector	DECTRIS PILATUS3 X 1M
Wavelength(s)	0.994
Spacegroup name	I 2 3
Unit cell lengths	360.661, 360.661, 360.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.700 - 3.410
Rwork	0.339
R-free	0.33900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nap
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	GLRF
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.700	3.450
High resolution limit [Å]	3.410	3.410
Rmerge		1.260
Number of reflections	92015
<I/σ(I)>	5.6	0.4
Completeness [%]	87.4	43.7
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	NaCitrate pH 6.5, 5% (v/v) PEG-4,000, 0.2M NDSB-2