5J8Y
Crystal structure of the Scm-SAM and Sfmbt-SAM heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.956, 53.970, 61.455 |
| Unit cell angles | 90.00, 109.22, 90.00 |
Refinement procedure
| Resolution | 47.170 - 1.980 |
| R-factor | 0.2558 |
| Rwork | 0.255 |
| R-free | 0.27720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pk1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.706 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1678) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.170 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.136 | 0.870 |
| Number of reflections | 21180 | |
| <I/σ(I)> | 11.21 | 1.91 |
| Completeness [%] | 96.9 | 83.18 |
| Redundancy | 9.1 | 6.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.05M Tris/HCl pH 7.5, 4% MPD, 0.2M ammonium acetate, 32.5% PEG3350 |
