5J8J
A histone deacetylase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-22 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 104.974, 104.974, 86.964 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.284 - 2.716 |
| R-factor | 0.1922 |
| Rwork | 0.190 |
| R-free | 0.23820 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.281 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.700 | |
| Rmerge | 0.086 | |
| Number of reflections | 14972 | |
| <I/σ(I)> | 14.7 | 3.1 |
| Completeness [%] | 98.1 | 96.9 |
| Redundancy | 10 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 285 | 0.1 M Sodium acetate trihydrate pH 4.6, 2.0 M Sodium formate |
