5J8J
A histone deacetylase from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-BM |
Synchrotron site | APS |
Beamline | 17-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-22 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9792 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 104.974, 104.974, 86.964 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.284 - 2.716 |
R-factor | 0.1922 |
Rwork | 0.190 |
R-free | 0.23820 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.281 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.700 | |
Rmerge | 0.086 | |
Number of reflections | 14972 | |
<I/σ(I)> | 14.7 | 3.1 |
Completeness [%] | 98.1 | 96.9 |
Redundancy | 10 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 285 | 0.1 M Sodium acetate trihydrate pH 4.6, 2.0 M Sodium formate |