5J8E

Crystal structure of human Hook3's conserved Hook domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.12
Spacegroup name	P 2 21 21
Unit cell lengths	33.920, 75.880, 111.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.019 - 1.700
R-factor	0.1854
Rwork	0.184
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wix
RMSD bond length	0.006
RMSD bond angle	0.912
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.019	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.076	1.952
Number of reflections	32558
<I/σ(I)>	15.36	1.1
Completeness [%]	99.7	99.8
Redundancy	7	7.1
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2 M sodium formate, 0.1 M sodium acetate pH 4.6