5J8E
Crystal structure of human Hook3's conserved Hook domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.12 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 33.920, 75.880, 111.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.019 - 1.700 |
| R-factor | 0.1854 |
| Rwork | 0.184 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wix |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.912 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.019 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.076 | 1.952 |
| Number of reflections | 32558 | |
| <I/σ(I)> | 15.36 | 1.1 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 7 | 7.1 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2 M sodium formate, 0.1 M sodium acetate pH 4.6 |
