5J8A
Structure of the E coli 70S ribosome with the U1052G mutation in 16S rRNA bound to tigecycline
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 211.720, 435.190, 622.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.530 - 3.100 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.19200 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.820 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER-TNT (2.11.6) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.530 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 1027314 |
| <I/σ(I)> | 12.8 |
| Completeness [%] | 99.7 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 291 | PEG 8k, Spermidine, Putrescine, Ammonium Chloride, MAgnesium Chloride, MPD, MES pH 6.5 |
