5J81

Crystal structure of Glycoprotein C from Puumala virus in the post-fusion conformation (pH 6.0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97949
Spacegroup name	H 3 2
Unit cell lengths	96.430, 96.430, 247.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.215 - 1.800
R-factor	0.1841
Rwork	0.183
R-free	0.20880
Structure solution method	SIRAS
RMSD bond length	0.009
RMSD bond angle	0.893
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.900
High resolution limit [Å]	1.800	10.000	1.800
Rmerge	0.094	0.028
Number of reflections	41365
<I/σ(I)>	14.65	54.74	1.38
Completeness [%]	99.9	97.2	99.9
Redundancy	9.93
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289	12% PEG 2000 MME, 0.1 M MES pH 6.0, 0.2 M (NH4)2SO4