5J7T
Molecular Understanding of USP7 Substrate Recognition and C-Terminal Activation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 104.330, 196.730, 226.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.376 - 3.200 |
| R-factor | 0.2519 |
| Rwork | 0.250 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nb8 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.579 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 74.245 | 74.245 | 3.211 |
| High resolution limit [Å] | 3.200 | 14.815 | 3.200 |
| Rmerge | 0.128 | 0.076 | 0.670 |
| Rmeas | 0.144 | ||
| Rpim | 0.066 | ||
| Total number of observations | 91597 | ||
| Number of reflections | 19259 | ||
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 99.2 | 71.7 | 100 |
| Redundancy | 4.8 | 3.6 | 5 |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 289 | PEG 3350, 0.2M tri-potassium citrate |
