5J7N

Crystal structure of a small heat-shock protein from Xylella fastidiosa reveals a distinct high order structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.45860
Spacegroup name	P 43 2 2
Unit cell lengths	68.940, 68.940, 72.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.452 - 2.900
R-factor	0.2131
Rwork	0.212
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gla
RMSD bond length	0.010
RMSD bond angle	1.208
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.940	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.115	0.673
Number of reflections	4089
<I/σ(I)>	17.3	3.5
Completeness [%]	98.0	97.5
Redundancy	18.6	19.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES sodium salt pH 7.5, 0.3 M sodium citrate, 20% 2-propanol, 15% ethylene glycol