5J7E
hEAG PAS domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2009-12-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 213.974, 39.058, 106.802 |
| Unit cell angles | 90.00, 118.03, 90.00 |
Refinement procedure
| Resolution | 29.130 - 1.900 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z6c |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.973 |
| High resolution limit [Å] | 1.900 | 1.905 |
| Number of reflections | 59648 | |
| <I/σ(I)> | 4.14 | |
| Completeness [%] | 96.4 | |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 20 mM Tris-HCl, 200 mM NaCl, 5 mM DTT |
