5J75
Fluorogen Activating Protein AM2.2 in complex with ML342
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.021, 72.415, 64.562 |
| Unit cell angles | 90.00, 99.53, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.000 |
| R-factor | 0.22378 |
| Rwork | 0.221 |
| R-free | 0.26295 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j74 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.617 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.641 |
| Number of reflections | 31546 | |
| <I/σ(I)> | 15.1 | 2.4 |
| Completeness [%] | 96.7 | 95 |
| Redundancy | 6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% PEG 3350 0.2M sodium nitrate |
