5J6Y
Crystal structure of PA14 domain of MpAFP Antifreeze protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2015-09-15 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.220, 50.550, 79.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.710 - 1.030 |
R-factor | 0.156 |
Rwork | 0.155 |
R-free | 0.16400 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.306 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.220 | 1.050 |
High resolution limit [Å] | 1.030 | 1.030 |
Rmerge | 0.042 | |
Number of reflections | 74600 | |
<I/σ(I)> | 23.9 | |
Completeness [%] | 83.2 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 20% PEG 3350, 0.2 M Calcium Chloride, Glucose, HEPES pH7 |