5J6R
Crystal structure of Human Papillomavirus Type 59 L1 pentamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.242, 161.743, 154.729 |
| Unit cell angles | 90.00, 110.34, 90.00 |
Refinement procedure
| Resolution | 49.697 - 4.011 |
| R-factor | 0.244 |
| Rwork | 0.243 |
| R-free | 0.26710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r5i |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.695 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.070 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.854 | |
| Number of reflections | 44094 | |
| <I/σ(I)> | 7.8 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 0.2 M magnesium acetate, 0.1 M sodium cacodylate, pH 6.5, 10% PEG 8000 |
