5J6P

Crystal Structure of Mis18(17-118) from Schizosaccharomyces pombe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 31 1 2
Unit cell lengths	120.568, 120.568, 73.702
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.660 - 2.600
R-factor	0.22849
Rwork	0.225
R-free	0.29436
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	2.020
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.390	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.095	0.506
Number of reflections	18911
<I/σ(I)>	14.9	4.8
Completeness [%]	99.8	100
Redundancy	10.6	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	0.2M (NH4)2SO4, 20% PEG 3350, 0.4M 1,6-Hexanediol, 0.1M Tris pH 8.5, 4% Dioxane