5J6G
Recognition of the MHC class Ib molecule H2-Q10 by the natural killer cell receptor Ly49C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9436 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 101.010, 57.800, 210.704 |
| Unit cell angles | 90.00, 96.15, 90.00 |
Refinement procedure
| Resolution | 46.160 - 3.300 |
| R-factor | 0.2224 |
| Rwork | 0.221 |
| R-free | 0.25320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.830 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.160 |
| High resolution limit [Å] | 3.300 |
| Number of reflections | 18559 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 12% Peg8000 10% Peg400 0.1M HEPES ph7.2 0.2M NaCl |
