5J69
Structure of Astrotactin-2, a conserved vertebrate-specific and perforin-like membrane protein involved in neuronal development
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9500 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 98.700, 86.600, 108.360 |
Unit cell angles | 90.00, 111.74, 90.00 |
Refinement procedure
Resolution | 60.770 - 3.630 |
R-factor | 0.2807 |
Rwork | 0.280 |
R-free | 0.30170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j67 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.669 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2283: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.770 | |
High resolution limit [Å] | 3.630 | 3.630 |
Rmerge | 0.188 | 1.547 |
Number of reflections | 9653 | |
<I/σ(I)> | 19.3 | |
Completeness [%] | 99.4 | |
Redundancy | 49.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Citric buffer pH 4.0 to 4.5 20% PEG 1000 |