5J69

Structure of Astrotactin-2, a conserved vertebrate-specific and perforin-like membrane protein involved in neuronal development

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9500
Spacegroup name	C 1 2 1
Unit cell lengths	98.700, 86.600, 108.360
Unit cell angles	90.00, 111.74, 90.00

Refinement procedure

Resolution	60.770 - 3.630
R-factor	0.2807
Rwork	0.280
R-free	0.30170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j67
RMSD bond length	0.003
RMSD bond angle	0.669
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((dev_2283: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.770
High resolution limit [Å]	3.630	3.630
Rmerge	0.188	1.547
Number of reflections	9653
<I/σ(I)>	19.3
Completeness [%]	99.4
Redundancy	49.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Citric buffer pH 4.0 to 4.5 20% PEG 1000