5J68
Structure of Astrotactin-2, a conserved vertebrate-specific and perforin-like membrane protein involved in neuronal development
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 103.900, 103.900, 304.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.802 - 5.221 |
| R-factor | 0.3483 |
| Rwork | 0.348 |
| R-free | 0.36450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j67 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.841 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2283: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.802 | |
| High resolution limit [Å] | 5.220 | 5.220 |
| Rmerge | 0.120 | 3.047 |
| Number of reflections | 6915 | |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 23.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES, pH 5.0 to 6.0, 5% PEG 6000 |
