5J63
Crystal Structure of the N-terminal N-formyltransferase Domain (residues 1-306) of Escherichia coli Arna in Complex with UDP-Ara4N and Folinic Acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-12 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.99768 |
Spacegroup name | P 1 |
Unit cell lengths | 75.994, 76.206, 84.852 |
Unit cell angles | 89.94, 63.78, 61.54 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.21571 |
Rwork | 0.212 |
R-free | 0.28404 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bln |
RMSD bond length | 0.013 |
RMSD bond angle | 1.685 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.550 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.056 | 0.144 |
Number of reflections | 45712 | |
<I/σ(I)> | 28.5 | 11.8 |
Completeness [%] | 91.3 | 88.7 |
Redundancy | 2.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 20 to 22% poly(ethylene glycol) 5000, 50 mM MgCl2, 5 mM N5-formyltetrahydrofolate, 10 mM UDP-Ara4N, and 100 mM HEPPS (pH 8.0) |