5J5C
Crystal structure of ARL1-GTP and DCB domain of BIG1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9777 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 107.819, 107.819, 204.627 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.400 |
| R-factor | 0.2267 |
| Rwork | 0.225 |
| R-free | 0.25810 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.128 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 7.320 | 3.400 |
| Rmerge | 0.107 | 0.049 | 0.488 |
| Number of reflections | 8735 | ||
| <I/σ(I)> | 3.3 | ||
| Completeness [%] | 85.0 | 97 | 30 |
| Redundancy | 6.2 | 5.6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.1 | 289 | 0.2M lithium sulfate, 0.1M sodium citrate tribasic, 0.1M Tris-HCl pH 9.1, and 27% (w/v) polyethylene glycol 3350 |
