5J5B
Structure of the WT E coli ribosome bound to tetracycline
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 212.310, 434.580, 624.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.160 - 2.800 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.21900 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BUSTER |
Refinement software | BUSTER-TNT (2.11.6) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.160 |
High resolution limit [Å] | 2.800 |
Number of reflections | 1291300 |
<I/σ(I)> | 188.6 |
Completeness [%] | 92.1 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 291 | PEG 8000, Spermidine, Putrescine, Ammonium Chloride, Magnesium Chloride, MES pH 6.5 |