5J58
Trypanosoma brucei methionyl-tRNA synthetase in complex with inhibitor (Chem 1856)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-26 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.970, 106.030, 207.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.700 - 2.800 |
R-factor | 0.20296 |
Rwork | 0.202 |
R-free | 0.22986 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.347 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.700 |
High resolution limit [Å] | 2.800 |
Number of reflections | 47346 |
<I/σ(I)> | 7.6 |
Completeness [%] | 98.7 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1.8-2.1 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M sodium cacodylate, pH 6.2-6.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |