5J4G

Crystal structure of the C-terminally His6-tagged HP0902, an uncharacterized protein from Helicobacter pylori 26695

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-12-12
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	50.355, 50.355, 142.031
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.076 - 2.600
R-factor	0.1856
Rwork	0.182
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q30
RMSD bond length	0.011
RMSD bond angle	1.354
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	71.000
High resolution limit [Å]	2.500
Rmerge	0.042
Number of reflections	6158
<I/σ(I)>	39.9
Completeness [%]	98.2
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	PEG 3350, ammonium acetate, bis-tris