5J45
Crystal structure of Shrub, fly ortholog of SNF7/CHMP4B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 43 |
Unit cell lengths | 29.767, 29.767, 174.311 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.578 - 2.758 |
R-factor | 0.2212 |
Rwork | 0.216 |
R-free | 0.26880 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.583 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1951) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.758 | 2.758 |
Rmerge | 0.090 | |
Number of reflections | 3869 | |
<I/σ(I)> | 15.9 | |
Completeness [%] | 98.1 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 17% v/v PEG10K, 0.1 M NH4CH3CO2, and 100 mM Bis-Tris pH 5.5 |