5J40

The X-ray structure of JCV Helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.999971
Spacegroup name	P 6
Unit cell lengths	109.910, 109.910, 67.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.970 - 2.170
R-factor	0.172
Rwork	0.169
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.000
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.3.0)
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.972	38.972	2.290
High resolution limit [Å]	2.170	6.880	2.170
Rmerge		0.022	0.446
Number of reflections	22759
<I/σ(I)>	20.6	28.1	1.7
Completeness [%]	92.0	85.9	94.7
Redundancy	5.7	5.6	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	25-30% Peg400, 0.1 M LiSO4, 0.1M HEPES (pH 7.0), 0.2 M NaCl