5J3B

Structure of translation elongation factor P from Acinetobacter baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-30
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	130.760, 52.230, 90.170
Unit cell angles	90.00, 130.62, 90.00

Refinement procedure

Resolution	45.027 - 2.300
R-factor	0.1975
Rwork	0.193
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a5z
RMSD bond length	0.008
RMSD bond angle	0.969
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_2356)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.027	50.000	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.042	0.031	0.557
Number of reflections	20641
<I/σ(I)>	20.33	38.45	2.88
Completeness [%]	98.7	70	99.5
Redundancy	5
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	290	Microlytic MCSG1 screen D4: 20% PEG 3350, 200mM KSCN; AcbaC.17944.a.B1.PW37730 at 18mg/ml; cryo: 15% EG ; tray 265235d4, puck ecq0-81