5J34
Isopropylmalate dehydrogenase K232M mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.803, 76.820, 209.497 |
Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
Resolution | 38.410 - 1.827 |
R-factor | 0.1664 |
Rwork | 0.165 |
R-free | 0.18550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | R8W |
RMSD bond length | 0.007 |
RMSD bond angle | 0.996 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.500 | 1.860 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.642 | |
Number of reflections | 211778 | |
<I/σ(I)> | 28.6 | 2 |
Completeness [%] | 98.8 | 99.4 |
Redundancy | 3.4 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.16 M ammonium sulfate, 0.07 M sodium acetate, 17.5% (v/v) PEG-4000, and 20% (v/v) glycerol |