5J30

Thermus thermophilus 70S termination complex containing E. coli RF1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	210.030, 449.710, 620.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.807 - 3.200
R-factor	0.2156
Rwork	0.214
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.003
RMSD bond angle	0.737
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.810		3.280
High resolution limit [Å]	3.200	14.310	3.200
Rmerge	0.280	0.046	2.652
Number of reflections	955012
<I/σ(I)>	8.16	35.9	1.17
Completeness [%]	99.9	96.4	99.9
Redundancy	10.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
2	VAPOR DIFFUSION, SITTING DROP	7	293	4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC