5J2T
Tubulin-vinblastine complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.250, 157.740, 182.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.168 - 2.200 |
R-factor | 0.1959 |
Rwork | 0.193 |
R-free | 0.24400 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4i4t |
RMSD bond length | 0.008 |
RMSD bond angle | 1.074 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.200 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 150857 | |
<I/σ(I)> | 11.9 | 0.71 |
Completeness [%] | 97.8 | 92 |
Redundancy | 12.9 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 6% PEG, 12% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1M MES/0.1M imidazole |