5J2S
NKR-P1B from Rattus norvegicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 32 |
| Unit cell lengths | 60.721, 60.721, 76.589 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.590 - 2.000 |
| R-factor | 0.22134 |
| Rwork | 0.221 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yhf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.813 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.590 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.078 | 0.702 |
| Number of reflections | 21405 | |
| <I/σ(I)> | 21.8 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 80 mM HEPES, pH 7.5, 20% (w/v) PEG3000, 20% (v/v) glycerol |
