5J2J
Ternary complex crystal structure of DNA polymerase Beta with T:G mismatch at the primer terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-16 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.820, 80.170, 55.510 |
| Unit cell angles | 90.00, 107.85, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| Rwork | 0.201 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.111 | 0.444 |
| Number of reflections | 20909 | |
| <I/σ(I)> | 9.5 | 2.7 |
| Completeness [%] | 96.5 | 94.4 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 16-18% PEG 3350, 50 mM Imidazole, 350 mM Sodium Acetate |
